The invention of PCR in the mid-80s revolutionized molecular biological research, particularly with the continuous development of many innovative applications of PCR-related technologies in the field ...

Here, we describe a method of systematic PCR screening with multiround sample pooling for the isolation of rare PCR-positive samples. As an example, we have applied this protocol to the recovery of ...

My cry of frustration echoed down the hall. My latest attempt to subclone five important PCR products had failed yet again. After two months of fruitless effort, I was at my wit's end until a ...

PCR testing works by creating copies of a particular section of DNA, but there’s no evidence that PCR tests are being used or would be used to clone entire humans. PCR tests are effective at testing ...

GenScript Corporation has launched a new product geared toward its gene cloning customers. The CloneEZ ™ kit can clone any insert into any part of any vector. The traditional cloning process requires ...

Colony picking is a very labor-intensive task that is generally performed during cloning protocols, alongside additional upstream and downstream processes. Manual colony picking is both slow and ...

Researchers often call the polymerase chain reaction test – known as the PCR test – "the gold standard" for detecting the COVID-19 virus. The test is considered highly reliable and effective, and it ...

This is a preview. Log in through your library . Abstract Cellulose biodegradation is based upon the action of different microbial enzymes such as β-1,4-endoglucanases, exoglucanases, and ...

As part of a project to identify symbiosis-related genes, we report here a simple differential screening procedure for isolating up- and down-regulated fungal transcripts from a cDNA library of the ...

International contract research organization GenScript Corporation has launched a new product geared toward its gene cloning customers. The CloneEZ ™ kit can clone any insert into any part of any ...